Preservation of Bovine Spermatozoa at −79 and −196° C

O-3: In-Situ Preservation of Caprine CaudaEpididymal Spermatozoa at -10 Degree Centigrade

Background: In-situ preservation of cauda epididymal spermatozoa at -10 degree centigrade with electrolyte free media for obtaining maximum functional gametes than preservation at 5 degree centigrade. Materials and Methods: Electrolyte free media prepared with soybean lecithin-glycerol, Coenzyme Q10 - glycerol and soybean lecithin - Coenzyme Q10- glycerol were inoculated separately into ligated...

Freeze-fracturing in ultrahigh vacuum at -196 degrees C

Conventional freeze-etching is carried out in a vacuum of approximately 10(-6) torr and at a specimen temperature of -100 degrees C. The relatively poor topographic resolution of most freeze-etch replicas, and the lack of complementarity of morphological details in double replicas have been thought to be caused by structural distortions during fracturing, and radiation damage during replication...

Phospholipids of bovine spermatozoa and seminal plasma.

Spermatozoal and seminal plasma lipids of fourteen individual bulls were separated by column chromatography into neutral lipid and several phospholipid fractions. Elution progress was monitored by thin-layer chromatography. Each phospholipid constituent was determined by phosphorus analysis. Total lipid, cholesterol and plasmalogen contents were determined. The fatty acids and aldehydes of the ...

The Viability and Fertility of Bovine Spermatozoa

Ghrelin enhances viability of rat spermatozoa during incubation at 37°C

Antioxidant properties of ghrelin have been demonstrated in recent studies. In the present study, theeffects of chronic administration of ghrelin on the motility and plasma membrane integrity of rat spermatozoa during incubation at 37ºC were investigated. Thirty 45-day-old male Wistar rats were divided into control and treatment groups. Rats in the treatment group were daily injected subcutaneo...